Isolation and identification bacteria causing oral infection from women above 55 years and study their relation to some immunological factors

The mouth cavity is a vital component of human body as well as a little environment. The existence of healthy native microflora on gum, teeth, and linings of an individual's oral cavity is critical to that person's ability to maintain good oral health To isolate and identify bacteria causing oral infection from women upper 55 years. During the time period of November 2022 to January 2023, a total of 119 oral swabs were collected from patients at the Baghdad Teaching Hospital, Children Hospital who had been diagnosed by medical professionals as having oral infection symptoms. The patients included women whose ages ranged from >55 years, and information was taken from each review, including the age, residence, and underlying diseases. Microorganisms that were grown in pure culture were analyzed for their morphological properties on selective and differential medium in order to determine their identities. The color, shape, size, edge, and height of the growing colonies were used to make a diagnosis of the bacteria that were responsible for the growth. All operations were carried out in accordance with the instructions provided by the manufacturer as well as the standard operating procedures of microbiological laboratories. The study found that S. aureus was the most isolated bacteria with rate 27.33%, followed by Viridians Streptococcus (26.05%), S. mutans (17.65%), Streptococcus pyogenes (14.29%), and lowest rates were for S. pneumonia and E. coli.  In this study, all S. aureus isolates were sensitive to Amikacin, 90.9% to Cefixime, 63.64% to Gentamicin, 54.55% to Amoxicillin+ clavulanic acid, 42.42% to Cefotaxime, 33.33% to Ampicillin and 30.3% for each of Chloramphenicol, Erythromycin and Cephalothin while all isolates were resistant to Penicillin. S. pyogenes isolates were 100% sensitive to penicillin and ampicillin, 87.1% were sensitive to chloramphenicol, 48.39% to Cefotaxime, 41.94% to Cefixime, 32.26% to Gentamicin and most isolates were resistant to Amoxicillin+ clavulanic acid, Erythromycin, Amikacin and Cephalothin. The study demonstrated that all of S. mutans isolates were sensitive to Penicillin and Ampicillin, 85.71% to Cefotaxime, 66.67% to chloramphenicol, 42.86% Gentamicin and 38.1% to Cefixime while high rate were resistant to Amoxicillin+ clavulanic acid, Amikacin, Erythromycin and Cephalothin. The study showed that the highest mean of serum IgM, IgG and IgA was recorded among oral infection patients (15.19, 33.27 and 76.38 pg/ml respectively) as compared with healthy control group (0.17, 10.18 and 23.24 pg/ml respectively), the difference was highly significant at P. value 0.01. The study showed that the highest mean of Saliva IgM, IgG and IgA was recorded among oral infection patients (11.81, 26.17 and 118.3 pg/ml respectively) as compared with healthy control group (0.15, 7.34 and 44.27 pg/ml respectively), the difference was highly significant at P. value 0.01.