Journal ID : CJOHNS-25-04-2024-11441
[This article belongs to Volume - 55, Issue - 04]

Title : THE CYTOTOXIC CONSEQUENCES OF SEVERAL TOPICALLY APPLIED GELS COMPRISING HYALURONIC ACID (HA) ON ORAL MICROORGANISMS AND GINGIVAL FIBROBLASTS

Abstract :

The current study aims to find out the cytotoxic consequences of several topically applied gels comprising hyaluronic acid (ha) on oral microorganisms and gingival fibroblasts. The study was conducted from July to December 2023. It examined the effects of four different topical gels and a chlorhexidine gel on human gingival fibroblasts. The cells were treated with different concentrations of gels and DMEM, and their viability was tested. The morphology of the cells was assessed using phase-contrast microscopy. The optical density of the suspension was tested, and the presence or absence of colony growth was examined. The statistical software SPSS 28 was employed to evaluate the data. ANOVA was used for group comparisons, and a significance level of p < 0.05 was established. At doses of 0.782 percent and higher, none of the gels exhibited cytotoxicity throughout the 15-minute exposure period at 1-day viability. After three days of culture, the 15-minute category's vitality increased for all gel dosages, except BG and AlC, compared to the control. For the latter, we see a sharp decline in the total number of cells in all categories. AfC did not show any antibacterial impact after one minute of exposure, leaving only planktonic bacteria at a 50% concentration susceptible to antibacterial action. AlC outperformed S.oralis in its antibacterial activity, although it had a greater potential for cytotoxicity against HGF. The least cytotoxic and least effective against bacteria were BA and G. AfC showed minimal cytotoxicity and bacteriostatic properties on HGF.

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