Bleeding from extremity injuries is the most common cause of preventable death. The use of an arterial tourniquets as tools to aid hemostasis has been commonly used clinically in trauma or in other cases. This study aims to determine the effect of reperfusion interval on brain damage due to remote organ injury from Ischemic Reperfusion Injury with the use of tourniquets in long bone fractures. An experimental study using Randomized Complete Design was conducted on male white rats (Rattus novergicus). The experimental animals used were assumed to be homogeneous by going through several exclusion and inclusion criteria. In this study, there were 3 experiment groups, which were (P1 control) treatment in which tourniquets were not being given a reperfusion interval of 3 hours, (P2 control) treatment in which tourniquets with a reperfusion interval of 10 minutes, and (P3 control) treatment with tornickets with a reperfusion interval of 20 minutes, then the selection of rats treated with a reperfusion interval of 20 minutes, 10 minutes, and the control was conducted completely random. The variables which were measured were MDA levels, brain neuron cell histology, and brain neuron necrosis at reperfusion intervals using a tourniquet. The results showed that the MDA levels of rats in the intervention group were lower than in the control group (p<0.05) and the administration of reperfusion intervals decreased brain MDA levels by -34.072 μM/L while the duration of the reperfusion interval of 20 minutes was optimal in reducing brain MDA levels. In the histological study, necrosis of cerebrum cells (neurons) was not observable in all three groups and the thing which could be assessed was leukocyte cells. However, there was no significant difference in the number of leukocytes between the control and intervention groups. The duration of the reperfusion interval using a tourniquet at 10- and 20-minutes decreases brain MDA levels and leukocyte count.