Grafting is the act of removing fat from a donor to a recipient. The main disadvantage of this procedure is the high resorption of the fat graft or the low survival rate of the transplanted fat tissue. Several methods are implemented to increase the survival rate of fat grafts. Insulin is an option to increase the survival rate of fat grafts because insulin can increase the metabolic activity of fat cells, inhibit lipolysis, and stimulate fibroblasts. This study aimed to explore the effect of topical insulin on fat graft survival rates in a rabbit model. This research is an experimental study using Randomized Post Test Only Control Group Design on 16 (sixteen) male New Zealand rabbits (Oryctolagus cuniculus). The independent variable of this study was the application of long-acting insulin to adipose tissue prior to grafting. The dependent variables were weight maintenance and volume maintenance. Survival rates were calculated and analyzed using SPSS software. Results of this study Weight maintenance and volume maintenance in the experimental group were statistically higher than the control group (p <0.05). This study demonstrated differences in fat graft survival rates on insulin compared with normal saline. The conclusion is that in the rabbit model, insulin can improve the retention of adipose tissue grafts and significantly increase the survival rate of autotransplanted adipose tissue. Further studies with longer observation periods and other assessment methods are needed to better understand the mechanisms of fat tissue retention after grafting.